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Testing the DNA Quality from the FTA Card Sample Using the Chelex-Saponin Method.

Intro:
Diabetes Mellitus (DM) is a metabolic disorder characterized by abnormally high blood sugar levels (hyperglycemia) which are caused by insulin function problems, insulin anomalies, or a combination of the two. In Indonesia, particularly in isolated areas, genomic research on diabetes mellitus is relatively uncommon because of the difficulties of collecting whole blood DM samples from patients. One solution is to use the FTA Card to store blood samples in the form of a spot at room temperature. However, the DNA isolation results from the FTA card sample still have insufficient quality for advanced molecular analysis. The Chelex-Saponin method is one method for isolating DNA from the FTA card sample.

Aims:
This study aims to assess the quality of DNA recovered from the FTA card using the Chelex-Saponin method and then perform PCR amplification analysis on target genes associated with diabetes mellitus.

Methods:
Ten
samples of FTA cards were obtained from patients with type 2 diabetes mellitus. For PCR amplification, ABCC8, KCNJ11, and TCF7L2 specific primers were used.

Results:
The findings of this experiment established that the DNA genome of a patient with type 2 diabetes mellitus could be recovered successfully from FTA cards. Additionally, DNA can be used to amplify the ABCC8 (100%), KCNJ11 (60 %), and TCF7L2 genes (40 % ).

Conclusions:
The study shows that the Chelex-saponin technique can be utilized to extract DNA from diabetes patients’ blood samples collected on FTA cards and then analyzed genomically.

Source:
Maliza, R., Savitri, E., Arya, B., Faridah, N.I., Dania, H., Perwitasari, D.A., Jurnal Katalisator, Vol 6 No 1 (2021) 136-146.

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